质粒名称: | TRUPATH Triple Gai1 |
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出品公司: | Addgene |
目录编号: | 196048 |
存储实验室: | Justin English |
载体骨架基本信息 |
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载体名称: | pcDNA3.1(+) |
载体出品公司: | Genscript |
空载体大小: | 5140bp |
完整质粒大小: | 9868bp |
载体类型: | 哺乳细胞表达, Luciferase |
筛选标记: | 新霉素(G418) |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称1: | GAlphai1-RLuc8、GNAI1 |
物种来源: | H. sapiens (human) |
插入大小: | 2031bp |
基因突变: | RLuc8 and flanking SGGGGS linkers have been inserted at amino acid position 91 of the alpha subunit |
启动子: | CMV |
克隆方法: | 未知 |
5’克隆位点: | AgeI(未破坏) |
5’测序引物 | gccacgttgtgagttggatag |
3’测序引物 | TAGAAGGCACAGTCGAGG |
插入基因名称2: | GGamma9-GFP2、GNG9 |
物种来源: | H. sapiens (human) |
插入大小: | (bp)939 |
启动子: | CMV |
标签融合: | GFP2 and GSAG linker (N terminal on insert) |
克隆方法: | 未知 |
5’测序引物 | gagggcagaggaagtcttc |
3’测序引物 | GATTTAGGTGACACTATAG |
插入基因名称3: | GBeta3、GNB3 |
物种来源: | H. sapiens (human) |
插入大小: | (bp)1020 |
启动子: | CMV |
克隆方法: | 未知 |
5’测序引物 | CGCAAATGGGCGGTAGGCGTG |
3’测序引物 | ccggtagggccgggattc |
文献引用方法 |
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材料和方法部分: | TRUPATH Triple Gai1 was a gift from Justin English (Addgene plasmid # 196048 ; http://n2t.net/addgene:196048 ; RRID:Addgene_196048) |
参考文献部分: | TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT. Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 10.1038/s41589-020-0535-8. 10.1038/s41589-020-0535-8 PubMed 32367019 |
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TRUPATH Triple Gai1载体质粒基本信息
TRUPATH Triple Gai1质粒图谱载体图谱和TRUPATH Triple Gai1载体序列质粒序列多克隆位点信息
TRUPATH Triple Gai1质粒载体简介
TRUPATH Triple Gai1质粒序列载体序列
LOCUS TRUPATH Triple Gai1 10159 bp DNA circular SYN 25-AUG-2024 DEFINITION Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10159) AUTHORS Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT TITLE TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. JOURNAL Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 10.1038/s41589-020-0535-8. PUBMED 32367019 REFERENCE 2 (bases 1 to 10159) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 10.1038/s41589-020-0535-8." FEATURES Location/Qualifiers source 1..10159 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1937..1990 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" primer_bind complement(3007..3024) /label=SP6 /note="SP6 promoter, forward primer" misc_feature 3036..3609 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 3059..3611 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3203..3220) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3430..3449 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" primer_bind complement(5750..5767) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5756..5980 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6026..6454 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6113..6132) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6323..6344 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6463..6483) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6468..6797 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6648..6783 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6710..6729 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 6864..7658 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6918..6937) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7528..7547 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 7832..7953 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7869..7888) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7923..7942 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8002..8018) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8002..8018) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8015..8037) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8026..8042 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8050..8080) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8095..8116 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8233..8250) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8404..8992) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8484..8503) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9163..10023) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9786..9805 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10024..10128) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttatgg gagagatgga gcagctgagg caggaggcag agcagctgaa 961 gaagcagatc gccgacgcca gaaaggcctg cgcagatgtg accctggcag agctggtgag 1021 cggactggag gtggtgggaa gggtgcagat gaggaccagg agaacactga ggggccacct 1081 ggccaagatc tacgccatgc actgggccac agactctaag ctgctggtgt ccgcctctca 1141 ggacggcaag ctgatcgtgt gggatagcta taccacaaac aaggtgcacg caatccccct 1201 gcgcagctcc tgggtcatga cctgtgccta cgccccttcc ggaaatttcg tggcatgcgg 1261 aggactggat aacatgtgct ctatctataa tctgaagagc cgggagggca acgtgaaggt 1321 gtctagagag ctgagcgccc acaccggcta cctgtcttgc tgtcggttcc tggacgataa 1381 caatatcgtg acatctagcg gcgacaccac atgcgcactg tgggacatcg agaccggaca 1441 gcagaagaca gtgtttgtgg gccacacagg cgactgtatg tccctggccg tgtctccaga 1501 tttcaatctg tttatctccg gagcatgcga cgcatctgcc aagctgtggg atgtgcggga 1561 gggcacctgt agacagacct tcacaggcca cgagagcgac atcaacgcca tctgcttctt 1621 tcccaatggc gaggccatct gcacaggcag cgacgatgcc tcctgtaggc tgttcgacct 1681 gcgcgccgat caggagctga tctgcttttc tcacgagagc atcatctgtg gcatcacctc 1741 cgtggccttt agcctgtccg gcaggctgct gttcgcagga tatgacgact tcaactgcaa 1801 cgtgtgggac tccatgaagt ctgagagggt gggcatcctg agcggccacg ataatcgcgt 1861 gtcctgtctg ggagtgaccg cagacggaat ggcagtggcc acaggcagct gggattcctt 1921 tctgaagatc tggaacgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa 1981 tcccggccct accggtatgg tgtccaaggg agaggagctg ttcaccggag tggtgcctat 2041 cctggtggag ctggacggcg atgtgaatgg ccacaagttt agcgtgtccg gagagggaga 2101 gggcgacgca acctacggca agctgacact gaagttcatc tgcaccacag gcaagctgcc 2161 cgtgccttgg ccaaccctgg tgaccacact gtcctacggc gtgcagtgtt tttctaggta 2221 tcctgaccac atgaagcagc acgatttctt taagtctgcc atgccagagg gctacgtgca 2281 ggagcggaca atcttcttta aggacgatgg caactataag accagagccg aggtgaagtt 2341 cgagggcgac acactggtga acaggatcga gctgaagggc atcgacttta aggaggatgg 2401 caatatcctg ggccacaagc tggagtacaa ctataatccc cacaacgtgt atatcatggc 2461 cgataagcag aagaacggca tcaaggtcaa tttcaagatc cgccacaata tcgaggacgg 2521 cagcgtgcag ctggccgatc actaccagca gaacacccca atcggcgacg gacccgtgct 2581 gctgcctgat aatcactatc tgtttacaca gtctgccctg agcaaggacc ccaacgagaa 2641 gcgggatcac atggtgctgc tggagttcgt gaccgcagca ggcatcacac tgggcatgga 2701 tgagctgtac aagggcagcg ccggcaccat ggcacaggac ctgtccgaga aggatctgct 2761 gaagatggag gtggagcagc tgaagaagga ggtgaagaat acaagaatcc ccatcagcaa 2821 ggccggcaag gagatcaagg agtatgtgga ggcccaggcc ggcaacgacc ctttcctgaa 2881 gggcatccca gaggataaga atccctttaa ggagaagggc ggctgcatca tcagctgacc 2941 gcggcgtacg aggcctgcat gctccggacc tgcaggttcg aagtcgacag atctcaattg 3001 gggcccctat agtgtcacct aaataattcc gcccccccct ctccctcccc cccccctaac 3061 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc 3121 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg 3181 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg 3241 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc 3301 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa 3361 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa 3421 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta 3481 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg 3541 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca 3601 cgatgataat atgggctgca ccctgtccgc agaggataag gcagcagtgg agcggtctaa 3661 gatgatcgat cggaatctga gagaggacgg cgagaaggcc gccagagagg tgaagctgct 3721 gctgctggga gcaggagagt ctggcaagag caccatcgtg aagcagatga agatcatcca 3781 cgaggccggc tactctgagg aggagtgtaa gcagtacaag gccgtggtgt attctaacac 3841 aatccagagc atcatcgcca tcatcagggc aatgggaaga agcggaggag gaggctccat 3901 ggcatctaag gtgtatgacc ctgagcagag gaagcgcatg atcaccggac cacagtggtg 3961 ggcaaggtgc aagcagatga acgtgctgga ttcctttatc aattactatg actctgagaa 4021 gcacgccgag aacgccgtga tcttcctgca cggcaatgcc acaagctcct acctgtggag 4081 gcacgtggtg ccccacatcg agcctgtggc aaggtgtatc atccctgatc tgatcggcat 4141 gggcaagagc ggcaagtccg gcaatggcag ctataggctg ctggaccact acaagtatct 4201 gaccgcctgg ttcgagctgc tgaacctgcc aaagaagatc atctttgtgg gacacgactg 4261 gggagcagca ctggcattcc actacgccta tgagcaccag gatcgcatca aggccatcgt 4321 gcacatggag agcgtggtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga 4381 ggacatcgcc ctgatcaagt ctgaggaggg cgagaagatg gtgctggaga acaatttctt 4441 tgtggagacc gtgctgccta gcaagatcat gaggaagctg gagccagagg agttcgccgc 4501 ctacctggag ccctttaagg agaagggcga ggtgcggaga cccacactgt cctggccacg 4561 cgagatccca ctggtgaagg gaggcaagcc tgatgtggtg cagatcgtgc ggaactacaa 4621 tgcctatctg agagcctctg acgatctgcc aaagctgttc atcgagagcg accccggctt 4681 cttttccaat gccatcgtgg agggcgccaa gaagttccca aacaccgagt ttgtgaaggt 4741 gaagggcctg cactttctgc aggaggacgc ccccgatgag atgggcaagt acatcaagag 4801 cttcgtggag agagtgctga agaatgagca gtccggagga ggaggctctc tgaagatcga 4861 ctttggcgat tccgccaggg ccgacgatgc aaggcagctg ttcgtgctgg caggagcagc 4921 agaggagggc tttatgacag cagagctggc aggcgtgatc aagaggctgt ggaaggatag 4981 cggcgtgcag gcctgcttca accggtctag agagtaccag ctgaatgaca gcgccgccta 5041 ctatctgaac gacctggata gaatcgccca gcctaactat atcccaaccc agcaggacgt 5101 gctgaggaca cgcgtgaaga ccacaggcat cgtggagacc cacttcacct tcaaggatct 5161 gcacttcaag atgtttgacg tgggaggaca gaggtccgag cgcaagaagt ggattcactg 5221 cttcgagggc gtgaccgcca tcatcttttg tgtggccctg tctgactacg atctggtgct 5281 ggccgaggat gaggagatga atcggatgca cgagtctatg aagctgttcg atagcatctg 5341 taacaataag tggtttaccg acacatccat catcctgttc ctgaacaaga aggacctgtt 5401 tgaggagaag atcaagaaga gccccctgac catctgctac cctgagtatg ccggctccaa 5461 tacatacgag gaggctgccg cctatatcca gtgtcagttt gaggatctga acaagagaaa 5521 ggacaccaag gagatctata cccacttcac atgcgccacc gatacaaaga acgtgcagtt 5581 cgtgtttgat gccgtgacag acgtgatcat caagaacaat ctgaaggact gtggcctgtt 5641 ctgaggtacc gagctcggat ccactagtcc agtgtggtgg aattctgcag atatccagca 5701 cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg 5761 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 5821 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 5881 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 5941 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 6001 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 6061 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 6121 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 6181 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 6241 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 6301 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 6361 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 6421 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 6481 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 6541 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 6601 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 6661 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 6721 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 6781 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 6841 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 6901 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 6961 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 7021 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 7081 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 7141 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 7201 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 7261 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 7321 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 7381 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 7441 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 7501 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 7561 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 7621 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 7681 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 7741 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 7801 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 7861 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 7921 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 7981 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 8041 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 8101 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 8161 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 8221 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 8281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 8341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 8401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 8461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 8521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 8581 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 8641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 8701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8821 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 8881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 9001 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 9061 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 9121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 9181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 9241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 9301 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 9361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 9421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 9481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 9541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 9601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 9661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9781 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9841 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9901 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9961 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 10021 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 10081 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 10141 aaaagtgcca cctgacgtc //
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TRUPATH Triple Gai1质粒载体应用举例
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